Endocrine Abstracts

Arginine and other aminoacids have been reported to induce GH discharge, even if their site of action has not been clearly established. The aim of this study was to determine the effects of different culture media on GH secretion by pig pituitary cells. Pituitary cells from adult female pigs were cultured for 72 h in the following media: DMEM-F12 (Group A), DMEM-F12 SALTS (Group B) and DMEM-F12 ARGININE FREE (Group C), in standard culture condition (0-48 h: FCS 10%; 48-72 h: F...